Biochemistry – Question 9
In which type of chromatography, the proteins are bound to another substance?
a) Gel Chromatography
b) Paper Chromatography
c) Hydrophobic Chromatography
d) Affinity Chromatography
d) Affinity Chromatography
Affinity chromatography exploits the high selectivity of most proteins for their ligands.
In theory, only proteins that interact with immobilized ligand adhere.
Bound proteins are then eluted either by competition with soluble ligand or less selectively by disrupting protein ligand interactions using urea, guanidine hydrochloride, mildly acidic pH or high salt concentration.
Important point:
Enzymes may be purified by affinity chromatography.
Answer: Affinity Chromatography
Ref: Harper’s Illustrated Biochemistry, 31st Edition and Page-25-26
