Microbiology Question 10
Fildes technique is used for the culture of?
1. Clostridium perfringens
2. Clostridium tetani
3. Clostridium botulinum
4. Clostridium difficile
Show Answer
Answer : 2 Clostridium tetani
- Clostridium tetani grows on ordinary media.
- Growth can be improved by blood and serum.
- Because of marked tendency to swarm over the surface of the agar, surface colonies are difficult to obtain.
- After incubating anaerobically for 24 hours, if the water of condensation at the bottom of a slope of nutrient agar is inoculated with a mixed cell culture, subcultures from the top of the tube will yield a pure growth of tetanus bacillus.
- This is called Fildes technique.
Ref: Ananthanarayan and Paniker’s Textbook of Microbiology, 9th edition, p259, 260
Image credits: Centers for Disease Control and Prevention’s Public Health Image Library. http:// phil.cdc. gov/phil/details.asp?pid=6372
