Biochemistry – Question 4
In chromatography mass movements of the substances in due to:
a) Diffusion
b) Electrophoresis
c) Osmosis
d) Paper chromatography
Answer and Explanation
a) Diffusion
Chromatography
Chromatography is a method by which a mixture is separated by distributing its components between two phases.
The stationary phase remains fixed in place while the mobile phase carries the components of the mixture through the medium being used.
Important point:
Diffusion is the process whereby solute is transferred in a fluid from a point of high concentration to a point of lower concentration.
Diffusion is a concentration driven mass transfer process in chromatography.
Answer: Diffusion
Ref: Harper’s Illustrated Biochemistry, 31st Edition and Page-24
