NEET PG Medical – 2020 – Question – 77
Parents of the Child complains of assaulted by one of their relatives and anal pain in a child, On investigation test shows yellow iodine crystals with picric acid – what is the name of the test?
a) Florence test
b) Barberio’s test
c) Acid phosphatase test
d) Creatine Phosphokinase
Correct Answer – B
Answer: B. Barberio’s test
It detects spermine (secreted by the prostate) and uses an aqueous or alcoholic solution of picric acid.
A saturated aqueous or alcoholic solution of picric acid, when added to the spermatic fluid, produces yellow needle-shaped rhombic crystals of spermine picrate. The reaction probably depends on the presence of prostatic secretion.
The Acid Phosphatase Test: The prostatic secretion element of seminal fluid contains 500 to 1000 times greater acid phosphatase than any other body fluid. Human red cells, semen of higher apes, and juice of cauliflower have acid phosphatase level similar to that of human semen.
THE ESSENTIALS OF FORENSIC MEDICINE AND TOXICOLOGY
By Dr. K.S. NARAYAN REDDY 33/e- pg-434
